Silver nanoparticles in cancer
The early detection and treatment of cancer are the basic problems faced by the cancer specialists and hence this area has attracted a great attention. One important point in the effectiveness of anti-cancer drugs is related to the possibility of reaching the target site in sufficient concentration and to the efficient activity without causing damage to healthy tissues and cells (Misra et al. 2010; Seigneuric et al. 2010).
In this direction, nanotechnology represents, at the moment, one of the new technologies with possibility to enhance the diagnosis and treatment of cancer. This could be achieved through new imaging agents, multifunctional targeted devices capable of bypassing biological barriers to deliver therapeutic agents directly to the biological target involved in cancer, nano-biosensors for predicting the disease and minimizing the growth of cancer cells and reducing the cost of treatments (Jain 2010; Qiao et al. 2010). Metallic nanoparticles, in this area, appear as important agents, since they are used in several biomedical applications, such as highly sensitive diagnostic assays and biosensors (Rai et al. 2012), thermal, and radiotherapy enhancement (Qiao et al. 2010), as well as drug and gene delivery with relatively low toxicity (Bhattacharyya et al. 2012; Conde et al. 2012). It is known that AgNPs interact with cells and intracellular macromolecules like proteins and DNA, probably through ROS, showing apoptotic bodies and necrotic cell death due to the cytotoxicity of biogenic AgNPs, although all of these possibilities are still under studies (Duran et al. 2010; Jeyaraj et al. 2013a, b). Several factors influence toxicity of AgNPs, such as dose, time and size of the particles. Against MCF-7 cell culture, it was found that toxicity is dose-dependent and causes cellular damage in Human Epidermoid Larynx (Hep-2) cell line through ROS formation (Jacob et al. 2012). Biologically synthesized AgNPs from the leaf of Suaeda monoica on Hep-2 cells exhibited dose-dependent toxicity at the concentration studied (Satyavani et al. 2012).
AgNPs biogenically synthesized from Podophyllum hexandrum leaf extract showed a cytotoxicity and apoptotic effect, probably through caspace-cascade activation and loses of mitochondrial integrity (Jeyaraj et al. 2013b). Piao et al. (2011) reported that hydroxyl radicals released by the AgNPs attack cellular components including DNA, lipids and proteins to cause various kinds of oxidative damages. Jeyaraj et al. (2013a) using biogenic AgNPs from Sesbania grandiflora leaf extract also showed cytotoxic effect against MCF-7 cell lines inducing cellular damage in terms of loss of cell membrane integrity, oxidative stress and apoptosis. The authors suggested that in order to progress to clinical cancer treatment it is necessary to study the formulation and clinical trials to establish the nanodrug to treat cancer cells.
In a recent study, biogenic AgNPs from Vitex negundo leaf extract showed inhibition of proliferation of human colon cancer cell line HCT15. These results indicate that AgNPs may exert antiproliferative effects on colon cancer cell line by suppressing its growth, arresting the G0/G1-phase, reducing DNA synthesis and inducing apoptosis (Prabhu et al. 2013). Inoculation of Dalton’s lymphoma ascites cells in mice produced a tumour progression in the animals, whereas in the presence of AgNPs a substantial decrease in cancer cell numbers in tumour mice was observed through histopathologic analysis. Apparently, the effect of AgNPs in increasing mean survival time and life span depends on their ability to reduce tumour cell viability and induce cytotoxicity (Sriram et al. 2010). The hematologic parameters examined in the controls, tumour controls and tumour-treated mice showed the effect of AgNPs in reducing white blood cell and platelet counts in tumour-bearing mice compared with controls. These data highlight the non-toxic effect of AgNPs, which did not induce any alteration in hematologic parameters for treated mice in comparison with controls and, at the same time, led to effective control of white blood cells that possess the immunologic constituents of ascitic fluid. This was corroborated recently by De-Lima et al. (2012, 2013) in cytotoxic and genotoxic biogenic AgNPs studies.
Most organs of our body are carrying tumor cells with the potential to cause cancer. Fortunately high quality Colloidal Silver with nanoparticle size less than 10 nm and high Zeta Potential charge is able to inactivate these tumor cells and may awaken new stem cells. Taking our minimal daily requirement of 500 mcg of CS might protect us not only against cancer but also Alzheimers. There are now over 50 medical studies demonstrating silver to be effective against cancer cells. An excellent synopsis by Steve Barwick of 50 of the clinical studies on silver and cancer can be seen at
Colloidal Silver and Cancer: A Surprising Look!Best-selling author Steve Barwick is back with this information-packed sequel to his two previous books, The Ultimate Colloidal Silver Manual (547-pages) and Colloidal Silver Success Stories: (94 pages).His brand new 192-page soft cover book, Colloidal Silver and Cancer:
Silver is the world’s oldest known antibiotic. There’s written evidence that the ancient Egyptians made use of it; the ancient Greeks and Romans stored their water, wine, and other liquids in silver vessels to prevent spoiling and contamination; a silver-based drug was found to be just as effective—and far less toxic to normal cells than platinum.
Infectious Microbes: the Real Culprits Behind Today’s Most Debilitating Chronic Degenerative Diseases at https://thesilveredge.com/hidden-infections/
“Colloidal silver induced dose-dependent cytotoxic effect on breast cancer cells; Therefore, it can be suggested that colloidal silver treatment may be used as an alternative treatment against cancer”.
“Evidence that silver-based nanoparticles applied alone reduce the viability of human oral squamous cell carcinoma cells”
Silver Nanoparticles Exhibit the Dose-Dependent Anti-Proliferative Effect against Human Squamous Carcinoma Cells Attenuated in the Presence of Berberine
First dual-targeting nanoparticles lower cancer’s defenses and attack tumors, In cancer patients, there are two main lines of immunotherapy: One disables cancer cells’ ability to hide from the immune system, and the other recruits the body’s T cells to destroy tumors. Jonathan P. Schneck and colleagues wanted to see if they could combine these two tactics with one nanoparticle-based platform. Testing on mouse models of melanoma and colon cancer showed that animals injected with the nanoparticles lived longer than those that did not receive the nanoparticles, and their tumor growth was delayed or even reversed in some cases. That the nanoparticles were effective against two different cancers suggests that they could help treat a variety of tumor types.
Date:June 7, 2017Source:American Chemical Society
Sep. 3, 2015 — Giving cancer patients aspirin at the same time as immunotherapy could dramatically boost the effectiveness of the treatment, according to new research.
Copper-based nanomaterials can kill cancer cells in mice, tumours are sensitive to copper oxide nanoparticles- a compound composed of copper and oxygen. Once inside a living organism, these nanoparticles dissolve and become toxic. By creating the nanoparticles using iron oxide, the researchers were able to control this process to eliminate cancer cells, while healthy cells were not affected.,Date:January 9, 2020 Source: KU Leuven Scientists have succeeded in killing tumor cells in mice using nano-sized copper compounds together with immunotherapy. After the therapy, the cancer did not return.
- Prabhu D, Arulvasu C, Babu G, Manikandan R, Srinivasan P. Biologically synthesized green silver nanoparticles from leaf extract of Vitex negundo L. induce growth-inhibitory effect on human colon cancer cell line HCT15. Process Biochem. 2013;48:317–324. doi: 10.1016/j.procbio.2012.12.013. [CrossRef] [Google Scholar]
- Qiao W, Wang B, Wang Y, Yang L, Zhang Y, Shao P. Cancer therapy based on nanomaterials and nanocarrier systems. J Nanomater. 2010;796303:1–9. doi: 10.1155/2010/796303. [CrossRef] [Google Scholar]
- Sriram MI, Barath S, Kanth M, Kalishwaralal K, Gurunathan S. Antitumor activity of silver nanoparticles in Dalton’s lymphoma ascites tumor model. Int J Nanomed. 2010;5:753–762. [PMC free article] [PubMed] [Google Scholar]
- Misra R, Acharya S, Sahoo SK. Cancer nanotechnology: application of nanotechnology in cancer therapy. Drug Discov Today. 2010;15:842–850. doi: 10.1016/j.drudis.2010.08.006. [PubMed] [CrossRef] [Google Scholar]
- Copper-based nanomaterials can kill cancer cells in mice Scientists have succeeded in killing tumor cells in mice using nano-sized (colloidal) copper compounds together with immunotherapy. After the therapy, the cancer did not return.Date:January 9, 2020Source:KU Leuven https://www.sciencedaily.com/releases/2020/01/200109105513.htm
Aug. 24, 2018 — Scientists have taken important steps forward in the development of a cancer-targeting immunotherapy. Researchers developed a treatment in mice that destroys part of the tumor and stimulates the … read more